Department of Biological Sciences > Faculty & Staff > Microbiology >Shengde Zhou

Representative Publications

Patents
US patent No. 7,026,152 (issued on April 11, 2006). Methods and compositions for simultaneous saccharification and fermentations. L.O. Ingram and S. Zhou

New Zealand patent No. 516,345 (issued on June 8, 2004). Recombinant hosts suitable for simultaneous saccharification and fermentation. L.O. Ingram and S. Zhou

Publications
Grabar, T.B., S. Zhou, K. T. Shanmugam, L. P. Yomano, and L. O. Ingram. 2006. Methyal glyoxal bypass identified as the source of chiral contamination in L(+) and D(-) lactate produced by recombinant Escherichia coli. Biotechnology Lett. 28(10):1527-1535

Zhou S., K. T. Shanmugam, L. P. Yomano, T. B. Grabar, and L. O. Ingram. 2006. Fermentation of 12% glucose to 1.2 M lactate by Escherichia coli strain SZ194 using mineral salts medium. Biotechnology Lett. 28(9):663-670

Zhou S., T. B. Grabar, K. T. Shanmugam, and L. O. Ingram. 2006. Betaine tripled the volumetric productivity of D(-)-lactate by Escherichia coli strain SZ132 in mineral salts medium. Biotechnology Lett. 28(9):671-676

Zhou, S., L. Yomano, K.T. Shanmugam, and L.O. Ingram. 2005. Fermentation of 10% sugars (w/v) to D-lactic acid by engineered Escherichia coli B. Biotechnol. Lett. 27:1891-1896.

Shukla, V., S. Zhou, L.P. Yomano, K.T. Shanmugam, J.F. Preston and L.O. Ingram. 2004. Production of D(-)-lactic acid from sucrose and molasses. Biotechnol. Lett. 26:689-693.

Causey, T.B., S. Zhou, K.T. Shanmugam, and L.O. Ingram. 2003. Engineering the metabolism of Escherichia coli W3110 for the conversion of sugar to redox-neutral and oxidized products: Homo-acetate production. PNAS: 100:825-832.

Zhou, S., K.T. Shanmugam, and L.O. Ingram. 2003. Functional replacement of Escherichia coli D(-)-lactate dehydrogenase (ldhA) with L(+)-lactate dehydrogenase (ldhL) from Pediococcus acidilactici. Appl. Environ. Microbiol. 69:2237-2244.

Zhou, S., T.B. Causey, A. Hasona, K.T. Shanmugam, and L.O. Ingram. 2003. Production of optically pure D-lactic acid in mineral salts medium by metabolically engineered Escherichia coli W3110. Appl. Environ. Microbiol. 69:399-407.

Underwood, S.A., S. Zhou, T.B. Causey, L.P. Yomano, K.T. Shanmugam, and L.O. Ingram. 2002. Genetic changes to optimize carbon partitioning between ethanol and biosynthesis in ethanologenic Escherichia coli. Appl. Environ. Microbiol. 68:6263-6272.

Zhou, S., and L.O. Ingram. 2001. Simultaneous saccharification and fermentation of amorphous cellulose to ethanol by recombinant Klebsiella oxytoca SZ21 without supplemental cellulase. Biotechnol. Lett. 23:1455-1462.

Zhou, S., and L.O. Ingram. 2000. Synergistic hydrolysis of carboxymethyl cellulose and acid-swollen cellulose by two endoglucanases (EGY and EGZ) from Erwinia chrysanthemi. J. Bacteriol. 182:5676-5682.

Ingram, L.O., H.C. Aldrich, A.C. Borges, T.B. Causey, A. Martinez, F. Morales, A. Saleh, L.P. Yomano, S.W. York, J. Zaldivar, and S. Zhou. 1999. Enteric bacterial catalysts for fuel ethanol production. Biotechnol. Prog. 15:855-866.

Zhou, S., and L.O. Ingram. 1999. Engineering endoglucanase-secreting strains of ethanologenic Klebsiella oxytoca P2. J. Industrial Microbiol Biotechnol. 22:600-607.

Zhou, S., L.P. Yomano, A. Saleh, F.C. Davis, H.C. Aldrich, and L.O. Ingram. 1999. Enhancement of expression and apparent secretion of Erwinia chrysanthemi endoglucanase (encoded by celZ) in Escherichia coli B. Appl. Environ. Microbiol. 65:2439-2445.

Zhou, S., T.A. McCaskey, and J. Broder. 1996. Evaluation of nitrogen supplements for bioconversion of MSW to lactic acid. Appl. Biochem. Biotechnol. 57/58: 517-524.

McCaskey T.A., S. Zhou, S.N. Britt, and R.C. Strickland. 1994. Bioconversion of municipal solid waste to lactic acid by Lactobacillus species. Appl. Biochem. Biotechnol. 45/46:555-568

Research Interests

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The research in my lab focuses on metabolic/genetic engineering of microbial biocatalysts for production of biobased fuels and chemicals using renewable lignocelluloses such as corn residues as a feedstock. This work will entail a combination of approaches in microbial physiology, biochemistry, molecular biology and genetic engineering, and fermentation technology. Particularly, I am interested in the genetic regulation of carbon central metabolism. Using functional genomic (gene knockout and replacement), metabolic flux, microarray, and proteomic analysis tools, we can elucidate critical metabolic points in carbon central metabolism. Therefore, "optimal" metabolic pathways can be designed and engineered to enhance the carbon flow and the reducing power generation through the central metabolism for production of useful compounds such as fuel ethanol, hydrogen, organic acids, amino acids, electricity using microbial fuel cells, and antibiotics.