Department of Biological Sciences > Faculty & Staff > Cell Biology > John L. Å. Mitchell

Representative Publications

Mitchell, J.L.A., Thane, T.K., Sequeira, J.M., Marton, L.J. and Thokala, R. (2007) Antizyme and antizyme inhibitor activities influence cellular responses to polyamine analogs. Amino Acids (in press),

Mitchell, J.L.A., Thane, T.K., Sequeira, J.M. and Thokala, R. (2007) Unusual aspects of the polyamine transport system affect the design of strategies for use of polyamine analogues in chemotherapy. Biochemical J.Proceedings (in press),

Mitchell, J.L.A., Simkus, C.L., Thane, T.K., Tokarz, P., Bonar, M.M., Frydman, B., Valasinas, A.L., Reddy, V.K. and Marton, L.J. 2004. Antizyme induction mediates feedback limitation of the incorporation of specific polyamine analogs in tissue culture. Biochem. J. 384: 271-279.

Mitchell, J.L.A. (2003) Regulation of polyamine metabolism. in COST 922: Health Implications of Dietary Amines; Heather Wallace, ed. Vol. 1, 89-100.

Vyakaranam, K., Rana, G., Spielvogel, B.F., Mitchell, J.L.A., Li, S., Zheng, C. and Hosmane, N.S. 2002. Synthesis and crystal structure of 1,4-diaminobutyl-N,N-bis(cyanoborane): the first substituted-borane adduct of putrescine. Inorganic Chem. Commun. 5, 522-524.

Mitchell, J.L.A., Leyser, A., Holtorff, M.S., Bates, J.S., Frydman, B., Valasinas, A.L., Reddy, V.K. and Marton, L.J. 2002. Antizyme induction by polyamine analogues as a factor in cell growth inhibition. Biochem.J. 366, 663-672.

Babal, P., Ruchko, M., Campbell, C.C., Gilmour, S.P., Mitchell, J.L.A., Olson, J.W. and Gillespie, M.N. 2001. Regulation of ornithine decarboxylase activity and polyamine transport by agmatine in rat pulmonary artery endothelial cells. Journal of Pharmacology & Experimental Therapeutics, 296:372-377.

Mitchell, J.L.A. and Judd, G.G.(1998). Antizyme modifications affecting polyamine homeostasis. Biochemical Society Transactions 26, 591-595.

Mitchell, J.L.A., Rupert, J., Leyser, A., and Judd, G.G.(1998). Mammalian cell polyamine homeostasis is altered by the radioprotector WR1065. Biochem. J. 335, 329-334.

Mitchell, J.L.A., Judd, G.G., Leyser, A., and Choe, C.-Y.(1998). Osmotic stress induces variation in cellular levels of ODC-antizyme. Biochem.J. 329, 453-459.

Mitchell, J.L.A., Choe, C.Y., and Judd, G.G.(1996). Ornithine decarboxylase stability in HMOA and the DH23b cells is not due to post-translational truncation of a C-terminal recognition site. Biochem.J. 318, 879-882. 

Research Interests

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Regulation of normal mammalian cell growth, and abnormalities that produce tumors. In particular, I have been studying putrescine and the polyamines spermidine and spermine, which are ubiquitous organic cations that affect the structure and activity of multiple cell components. Abnormal levels of these compounds can induce either apoptosis or tumor growth. Maintenance of polyamine homeostasis is mediated by an unusual regulatory protein, antizyme (AZ). We are investigating the various mechanisms whereby a cell alters its antizyme activity, and thereby cellular concentrations of the polyamines.