Department of Biological Sciences > Faculty & Staff > Cell Biology > Barbara P. Johnson-Wint

Representative Publications

• Yasui, L.S., Ling-Indeck, L., Johnson-Wint, B., Fink, T.J. Molsen, D. Nuclear Structure changes in the radiation sensitive CHO mutant cell, XRS-5. Radiat. Res. 127:269-277; 1991.
• Thompson, K.P., Hanna, K.D., Waring, G.O., Gipson, I.K., Liu, Y., Gailitis, R.P., Johnson-Wint, B., Green, K. Current status of synthetic epikeratoplasty. Refract. Corneal Surg. 7:240-248; 1991.
  
• Thompson, K.P., Hanna, K.D., Gipson, I.K., Gravagna, P., Waring, G.O., Johnson-Wint, B. Synthetic epikeratoplasty in rhesus monkeys with human type IV collagen. Cornea 12:35-45; 1992.
• Hagen, K.B., Waring, G.O. and Johnson-Wint, B. 1997. Progressive nonulcerative paracentral keratolysis associated with elevated corneal metalloproteinases. Cornea 16:486-492.

ABSTRACTS:

• Trinkaus-Randall, V., Johnson-Wint, B., Banwatt, R.S., Gibbons, D., Capecchi, J. and H.M. Leibowitz. 1992. Healing of a porous polymer in cornea and matrix metalloproteinase involvement. Invest. Ophthalmol. Vis. Sci. (Supplement) 32: 889.
• Malouvier, A., Johnson-Wint, B., McGowan, K., Daunton, N. and E. Morey-Holton. 1994. Effects of 2G centrifugation on rat bone growth and tissue collagen content. J. Bone Miner. Res. 9: S378.
• Johnson-Wint, B.P., Grymes, R. and Malouvier, A. 1995. Collagen contraction by corneal, skin and tendon fibroblasts: mechanisms and force effects. Biorheology 32: 175-176.
• Johnson-Wint, B. and Cobb, M. 1998. Temperature may mediate vibration stimulation of collagen gel contraction by rat tendon fibroblasts and bone forming cells. Gravitational and Space Biology Bulletin 12: 36.
• Palm, M.J. and Johnson-Wint, B. 1998. Blocking of biotinylated proteins prior to use in the amplified alkaline phosphatase immun-blot detection. . Gravitational and Space Biology Bulletin 12: 15.
  
• Palm, M.J. and B.P. Johnson-Wint. 1999.  The effects of hindlimb unloading on certain integrins and actins in rat metatarsals.  Mol. Biol. Cell 10:  344a.
  
• Johnson-Wint, B.P.  1999.  Effect of load on collagen gel contraction by rat tendon fibroblasts and osteoblasts/osteocytes in vitro.  Mol. Biol. Cell 10:  347a.
  
• B. Johnson-Wint and B. Coon.  2005.  Fibroblast shape and organization changes within contracting load bearing collagen gels.  Mol. Biol.Cell 16:   686a

Research Interests

Tendon Fibroblasts Contract Collagen Gels Faster Than Osteocytes: Image of Replicate 100 µl Cell Populated Collagen Gels After 24 Hours of Contraction

Six Replicate Gels Populated with 105 Rat Tendon Fibroblasts

Six Replicate Gels Populated with 105 Rat Metatarsal Osteocytes

Current research is in two areas of morphogenesis:

1. the nature and role of cell-cell interactions regulating collagen degradation in vertebrate connective tissue formation, maintenance, repair and pathology, with special interest in cornea and skin, and
2. force effects including gravity on physical manipulation of collagen fibrils by tendon fibroblasts and osteoblasts to determine tissue shape and strength.