Annotation Control

Useful Web Sites for Annotation, and they all open in separate tabs

Primary Annotation Sites
CGAT SEED Viewer
for Ammonifex
 
 
Manatee
for B. megaterium
RAST SEED Viewer
for B. meg

Background Information and Literature Research Sites
Google
Wikipedia
Todar's Online Textbook of Microbiology
Medical Microbiology Textbook from the University of South Carolina. You probably want to go to Bacteriology
***PubMed

Useful resources on KEGG
KEGG Home page and table of contents
KEGG Organisms List of all organisms in KEGG, along with their 3 letter abbreviations
KEGG Pathways List of all pathways and subsystems diagrammed in KEGG
Manet A more colorful version of the KEGG maps, coded by phylogeny.

NCBI Resources
NCBI Home page
***NCBI Blast You probably want "protein blast", in the Basic Blast section.
NCBI PubMed Literature searaches
NCBI Taxonomy Browser Paste the taxon ID (the 6 numbers after the "fig|" into the box and get domain and phylum information.
CDD Conserved Domains database at NCBI, an alternative to HMMs

Other Useful Sites
***UniProt Good text search for gene names, symbols, organisms, etc. AND, good BLAST search. The database is Swiss-Prot and TrEMBL combined. Swiss-Prot entries are manually curated, while TrEmBL entries aren't. (Note: this is a beta version. The older version is here
Metacyc Biochemical pathways, an alternative to KEGG
PFAM HMM Search, with links to the families
InterPro Scans several different databases. (Also has the coolest "in progress" indicator)
Protein Data Bank (PDB) Structural Information about proteins
BRENDA Vast amounts of info about individual enzymes

Resources at the J. Craig Venter Institute (formerly known as TIGR)
TIGR CMR Comprehensive Microbial Resource. Lots of info
TIGR HMM Search tool Very useful, but a bit slow.
TIGR Fams List of all TIGR Fams by number, with links to info about each
TIGR Genome Properties Which gene functions should be present in various subsytems, according to TIGR

Multiple Sequence Alignment
MUSCLE
T-COFFEE
CLUSTAL-W I don't recommend this one: it is a very primitive algorithm compared to the others.

Specialized B. megaterium resources
B meg annotation progress site
B meg BLAST search Also, get DNA sequence by gene ID or chromosome position, translate DNA to protein
Search B meg annotation , or interconvert TIGR and RAST gene IDs