Free Web-based Primer/Probe Design Software Tools

Website Address

Primer3

http://www-genome.wi.mit.edu/cgi-bin/primer/primer3_www.cgi

IDT PrimerQuest

http://biotools.idtdna.com/Primerquest/

Oligonucleotide Properties Calculators

Zuker mfold

http://www.bioinfo.rpi.edu/applications/mfold/old/dna/form1.cgi

IDT OligoAnalyzer

http://biotools.idtdna.com/Analyzer/oligocalc.asp

RNAture

http://www.rnature.com/oligonucleotide.html

Oligo Houses

Biosearch Technologies

http://www.biosearchtech.com/products/feature/order.asp

IDT Technologies

http://www.idtdna.com/program/catalog/catalog.asp

Operon

http://www.operon.com/

Proligo

http://www.proligo.com/

Sigma-Genosys

http://www.sigma-genosys.com/oligo.asp

TriLink Biotechnologies

http://www.trilinkbiotech.com/products/oligo/oligo_synthesis.asp

Other Q- PCR Links

Gene Quantification site

http://www.gene-quantification.info/

Real-Time Primer Database

http://medgen.ugent.be/rtprimerdb/

Primer Bank (Harvard)

http://pga.mgh.harvard.edu/primerbank/

Miscellaneous

RefSeq (Reference sequence database)

http://www.ncbi.nih.gov/RefSeq/

Spidey (cDNA to genomic alignment tool)

http://www.ncbi.nlm.nih.gov/spidey/

All about Molecular Beacons

http://www.molecular-beacons.org/

General Molecular Biology Links and Tools

http://iubio.bio.indiana.edu/

TATAA Biocenter

http://www.tataa.com/