Distinguished Research Professor

Educational Background

Ph.D. 1987 A. Mickiewicz University, Poznan, Poland 

M.S. (Biology) 1976 A. Mickiewicz University, Poznan, Poland 

M.S. (Chemistry) 1972 A. Mickiewicz University, Poznan, Poland

Interests: Plant Molecular Biology; Molecular Virology.

Contact: Jozef Bujarski 
jbujarski@niu.edu
(815) 753-0601 
Office: MO 439, Lab: MO 314
Dept. of Biological Sciences
Northern Illinois University
DeKalb, IL 60115

Course / Lab Information

• Bios 423 - Virology

Representative Publications

Aleksandra Dzianott, Joanna Sztuba-Solinska, Anni Moore, and Jozef J. Bujarski. Null alleles of the RNA Interference Genes Affect RNA-RNA Recombination of Brome Mosaic bromovirus in Arabidopsis thaliana.  Submitted to Molecular Plant-Microbe Interactions.

Sztuba-Solinska J, Urbanowicz A, Figlerowicz M, Bujarski JJ. RNA-RNA 2011. Recombination in Plant Virus Replication and Evolution.  Annu Rev Phytopathol. Vol. 49.

Sztuba-Solinska J, Stollar V, Bujarski JJ. 2011. Subgenomic messenger RNAs: mastering regulation of (+)-strand RNA virus life cycle. Virology. 412(2):245-55

Sztuba-Solinska J, Dzianott A, Bujarski JJ. 2011. Recombination of 5’ subgenomic RNA3a with genomic RNA3 of Brome mosaic bromovirus in vitro and in vivo. Virology. 410(1):129-41.

Bujarski, J., 2008. BROMOVIRUSES (BROMOVIRIDAE). In R.G. Webster and A. Granoff (eds.), Encyclopaedia of Virology, Third Edition.
Bujarski, J. 2007. Genetic Recombination in Viruses. Review. Encyclopedia of Virology. 3rd Edition. 2008.

Bujarski, J. 2007. Bromoviridae. Review. Encyclopedia of Virology. 3rd Edition. 2008
Sztuba-Solinska J., Bujarski J.J. 2008. Insights into the single-cell reproduction cycle of members of the family bromoviridae: lessons from the use of protoplast systems. J Virol. 82:10330-40.

Sandoval C, Pogany J, Bujarski J., Romero J. 2008. Use of a defective RNA of broad bean mottle bromovirus for stable gene expression in legumes. Arch Virol. 153:1755-8.

Wierzchoslawski, R. and Bujarski, J.J. 2006. Efficient in vitro system of homologous recombination in brome mosaic bromovirus. J Virol. 80:6182-7.

Wierzchoslawski, R., Urbanowicz, A., Dzianott, A., Figlerowicz, M., and Bujarski, J.J. 2006. Characterization of a novel 5' subgenomic RNA3a derived from RNA3 of Brome mosaic bromovirus. J Virol. 80:12357-66.

Bujarski J. and Wierzchoslawski R. 2006. METHOD OF PRODUCTION AND APPLICATION OF THE RNA POLYMERASE PROTEIN FROM BROME MOSAIC BROMOVIRUS. Patent application.

Urbanowicz A, Alejska M, Formanowicz P, Blazewicz J, Figlerowicz M, Bujarski JJ. 2005.Homologous crossovers among molecules of brome mosaic bromovirus RNA1 or RNA2 segments in vivo. Journal of Virology 79:5732-42.

Wierzchoslawski R, Bujarski JJ. 2004. Efficient in vitro system of homologous recombination in brome mosaic bromovirus. J Virol. 80:6182-7.

Wierzchoslawski R, Bujarski JJ. 2004. Efficient in vitro system of homologous recombination in brome mosaic bromovirus. J Virol. 80:6182-7.

Dzianott, A., N. Rauffer-Bruyere, and J. J. Bujarski. 2001. Studies on functional interaction between brome mosaic virus replicase proteins during RNA recombination, using combined mutants in vivo and in vitro. J. Virology. In press.

Bruyere, A., M. Wantroba, S. Flasinski, A. Dzianott, and J. J. Bujarski. 2000. Frequent homologous recombination events between molecules of one RNA component in a multipartite RNA virus. J. Virology 74:4214-4219.

Nagy, P.D., Ogiela C., and Bujarski, J. J. (1999) Mapping sequences active in homologous recombination in brome mosaic virus: prediction of recombination hot-spots. Virology 254, 92-104.

Figlerowicz, M. and Bujarski, J. J. (1999). RNA recombination in brome mosaic virus, a model plus stranded RNA virus. Acta Biochemica Polonica. 36, 12-18. 

Bujarski, J. J. (1999) Molecular basis of genetic variability in plant RNA viruses. In "Molecular Biology of Plant Viruses." C. L. Mandahar, ed. Kluwer Acad. Publ.

Bujarski, J. J. (1999) Recombination of Viruses. In Encyclopedia of Virology, Second Edition. R. G. Webster, A. Granoff, the editors.

Bujarski, J. J. (1999) Co-translated (with A. Milos) into Polish a book entitled The Red Queen by Matt Ridley; ed. REBIS . 

Nagy, P. D. and Bujarski, J.J. (1998) Silencing homologous RNA recombination hot spots with GC-rich sequences in brome mosaic virus. J. Virol. 72, 1122-1130.

Figlerowicz, M., Nagy, P., Tang, N., Kao, C. C., and Bujarski, J. J. (1998) Mutations in the N-terminus of the brome mosaic virus polymerase affect genetic RNA-RNA recombination. J. Virol. 72, 9980-9989.

Bujarski, J. J. (1998) Bromovirus isolation and RNA extraction. In "Plant Virology Protocols: from virus isolation to transgenic resistance." Edited by G. D. Foster and S. C. Taylor, Humana Press.

Flasinski, S., A. Dzianott, J. Spear, J. Johnson, and J.J. Bujarski. 1997. Structure-based rationale for the rescue of systematic movement of brome mosaic virus by spontaneous second-site mutations in the coat protein gene. J. Virol. 71:2500-2504. 

Nagy, P., and J.J. Bujarski. 1997. Engineering of homologous recombination hot-spots with AU-rich sequences in brome mosaic virus. J. Virol. 71:3799-3810. 

Pogany, J., J. Romero, and J.J. Bujarski. 1997. Effect of 5Õ and 3Õ terminal sequences, overall length and coding capacity on the accumulation of defective-like RNAs associated with broad bean mottle virus. Virology 228:236-243. 

Figlerowicz, M., P. Nagy, and J.J. Bujarski. 1997. A mutation in the putative RNA polymerase gene inhibits nonhomologous but not homologous genetic recombination in brome mosaic virus. Proc. Natl. Acad. Sci. USA 94:2073-2078. 

Figlerowicz, M., and Bujarski, J. 1997. Genetic recombination in RNA viruses. Postepy Biochemii (Progress in Biochemistry) 43, 257-267. 

A review in Polish. Bujarski, J. J. 1997. Experimental systems of genetic recombination and defective RNA formation in RNA viruses: Part II. Seminars in Virology 8, 75-76.

Nagy, P.D. and Bujarski, J.J. 1998. Silencing homologous RNA recombination hot spots with GC-rich sequences in brome mosaic virus. J. Virol. 72, 1122-1130.

Figlerowicz, M., Nagy, P., Tang, N., Kao, C.C., and Bujarski, J. J. 1998. Mutations in the N-terminus of the brome mosaic virus polymerase affect genetic RNA-RNA recombination. J. Virol. (in press). 

Bujarski, J. J. 1998. Bromovirus isolation and RNA extraction. Plant Virology Protocols: from virus isolation to transgenic resistance. Edited by G.D. Foster and S.C. Taylor, Humana Press.

Bujarski, J. J. and Milos, A. Translated from English to Polish: a book of Matt Ridley The red queen: sex and evolution of human nature. pp. 1 - 500. In editorial preparation.

Research Interests

Study of the mechanisms of RNA-RNA recombination (homologous and non-homologous) that occur in RNA viruses by using Brome Mosaic Bromovirus (BMV) as a model system, from both the virus and the host stand points. The main subjects under study include the determination of viral RNA sequences supporting crossover events, the role of viral replicase (RdRp) complex in recombination and the involvement of host cellular genes and the cellular pathways (such as RNAi/PTGS) in recombination. Also, evaluation of the risk of recombination associated with the use of transgenic plants that carry viral transgenes is another area of interest.