The main focus of the core lab is real-time quantitative PCR (QPCR). This procedure uses DNA copies of RNA (cDNA) to measure transcription of different genes. This procedure is similar to conventional PCR, except the abundance of PCR product can be measured after each cycle, not just the last cycle. This information is used to determine the relative abundance of different transcripts compared to a reference gene. This procedure has been used to investigate the effects of mutations on the expression of other genes, as well as the effect of different inhibitors on gene expression. Many studies in different organisms are possible with this technology. I am using a Mx3000P instrument from Stratagene to perform these reactions.
The Core DNA Analysis Lab uses capillary electrophoresis for DNA sequencing and fragment analysis. Sequencing uses fluorescent dyes to label partial copies of DNA provided by NIU scientists. Typically the nucleotide sequence of plasmids or PCR products are determined. The same equipment is used for fragment analysis, but the PCR products themselves are labeled. An internal size standard allows for precise size determination of these products. This information is commonly used for genotyping studies to distinguish between closely related individuals or populations.